Your challenge

The successful candidate will study glyco-profiles in the tumor microenvironment of pancreatic cancer patients and will address the biological importance of these signatures in patients biopsies and in vitro human models.

Your main tasks and responsibilities are:

  • Transcriptomic analysis of glycosylation related genes in patients data-sets;
  • Multiplex immune fluorescent microscopy analysis of tumor biopsis;
  • Cytometric analysis of tumor tissues and immune organs by flowcytometry and mass-spectometry analysis (cytof) ;
  • Validation of human cell lines, generation CRISPR-Cas9 glycovariant cell lines and co-culturing with immune cells;
  • You will use state-of-the-art high-dimensional cytometry techniques, RNA-sequencing, multiplex immune fluorescence and will apply bioinformatics to analyze the data.

Your profile

We are looking for a highly motivated and enthusiastic researchers with:

  • A Master’s degree in a field related to biomedical sciences, (medical) biology, biochemistry or bioinformatics;
  • Affinity for immunology and cancer;
  • Interest in high-dimensional and single-cell technologies and pushing the boundaries of research through technology-driven applications, knowledge to use R, is a plus;
  • Knowledge on multiplex histocytometry;
  • High-dimensional data analysis and biostatistics is a plus;
  • Excellent communications skills in English, both written and verbal.

Your work environment

Within the department of Molecular Cell Biology and Immunology at AmsterdamUMC, location VUmc, our group studies the glyco-immune code in cancer as novel checkpoint inhibition to discover new inhibitory pathways in the tumor microenvironment. For a recent KWF project that was granted to us we seek for a PhD student that incorporates fundamental research questions in the analysis of patients material of pancreatic cancer patients and human 3D modelsystems with the ultimate goal to incorporate new diagnostics and treatment to the clinic.

You will work in a team with young and enthusiastic researchers, who have expertise in glyco-immunology, biochemistry, cell biology and cancer. Research is embedded in the Cancer Immunology program of the research institutes Cancer Center Amsterdam (CCA) and Amsterdam Immunity and Infection Institute (AI&II).

Research within the department of Molecular Cell Biology and Immunology is performed in a vibrant and international high competitive level environment within the O|2 Lab Building located near the hospital. The department consists of several research groups that study immunological processes at cellular and molecular level in health and disease.

The research group ‘Dendritc cell immunobiology’ focusses on posttranslational modifications in the tumor microenvironment such as glycosylation that initiates novel immune suppressive pathways. Our goal is to discover new targets for treatment and biomarkers for diagnosis, and resolve new immune inhibitory pathways. We study glycosylation patterns in PDAC in both human tissues and murine model systems. Our work is performed in close collaboration with clinical and preclincal departments such as Surgery, Pathology and Center of Experimental and Molecular Medicine in AmsterdamUMC.

We offer

Salary Scale OIO: 2422 tot 3103 euro gross when employed full-time (depending on qualifications and experience).

We offer an initial contract for 12 months that can be extended (after evaluation) for another 36 months. We also provide you with the opportunity to follow further training and development.

In addition we offer:
– Additional benefits include an 8.3% end-of-year bonus and 8% holiday pay.
– Pension is accrued at the ABP. VUmc contributes a large part of the premium (70%).
– In addition to excellent accessibility by public transport, we reimburse 75% of your OV travel costs (commuting costs).

For more information about our employment conditions, please visit our website.
For Dutch citizens it is mandatory to provide a VOG (Verklaring Omtrent Gedrag).

Additional information

Relevant publications:
RodrÍguez E, Schetters STT, van Kooyk Y. The tumour glyco-code as a novel immune checkpoint for immunotherapy. Nat Rev Immunol. 2018 Mar;18(3):204-211.

Unger WW, Mayer CT, Engels S, Hesse C, Perdicchio M, Puttur F, Streng-Ouwehand I, Litjens M, Kalay H, Berod L, Sparwasser T, van Kooyk Y.Antigen targeting to dendritic cells combined with transient regulatory T cell inhibition results in long-term tumor regression. Oncoimmunology. 2014 Oct 31;4(8):e970462.

Information and application

Don’t hesitate to call us!
If you’re interested about this position, you can contact prof. dr. Y. van Kooyk, via telephone number +3120 444 8080.
If you need more information about the application procedure, contact Brend de Jong, corporate recruiter at +3120 444 8704.

You can apply till 19 April 2020 by using the apply button.

Acquisition based on this vacancy is not appreciated.